Human TLE3 ELISA Kit
Sensitivity
11 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00048 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TLE3 ELISA kit is to be used to detect and quantify protein levels of endogenous TLE3. The assay recognizes human TLE3. A polyclonal antibody specific for TLE3 has been pre-coated onto the microwells. The TLE3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for TLE3 is added to detect the captured TLE3 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human TLE3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Plasma, Cell culture supernatants, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 11 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7090 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 97% | 82%-126% |
| Urine | 90% | 78%-131% |
| Cell culture supernatants | 106% | 83%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
Transducin-like enhancer of split 3 (TLE3), also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. TLE3 is a transcriptional repressor that interacts with a chromatin complex acting downstream of adenomatous polyposis coil (APC) and β-catenin in the Wnt pathway. TLE3 is expressed in most β-cells and a subset of other endocrine cell types in the pancreas. TLE3 is first identified as a candidate biomarker of taxane sensitivity in breast cancer in a large screen of candidate immunohistochemical (IHC) classifiers in a community cohort study. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
