Recombinant Human TIM-3 protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human TIM-3 protein Ser22-Arg200 (Accession# Q8TDQ0-1) with a rabbit IgG Fc tag at the C-terminus |
| 基因ID | 84868 |
| 蛋白编号 | Q8TDQ0-1 |
| 预测分子量 | 46.1 kDa |
| SDS-PAGE | 52-72 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection. TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity. TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3.
参考文献:
1. Isabel Sada-Ovalle. et al.(2012) Nat Immunol.6(12):1245-52. 2. Chen Zhu. et al.(2005) Nat Immunol. 6(12):1245-52. 3. Mi Jin Lee. et al.(2009) Immune Netw.9(2):58-63. 4. Philipp S van de Weyer. et al.(2006) Biochem Biophys Res Commun. 351(2):571-6. 5. J J McIntire. et al.(2001) Nat Immunol. 2(12):1109-16.
