Recombinant Human Semaphorin 4D/CD100 protein (rFc Tag)(HPLC verified)
种属
Human
纯度
>90 %, SDS-PAGE
>90 %, SEC-HPLC
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human Semaphorin 4D protein Met22-Arg734 (Accession# Q92854-1) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 10507 |
| 蛋白编号 | Q92854-1 |
| 预测分子量 | 105.4 kDa |
| SDS-PAGE | 110-150 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.
参考文献:
1. Ewe Seng Ch'ng, et al. (2010) Mol Cancer. 9:251. 2. John R Basile, et al. (2007) J Biol Chem. 282(9):6899-905. 3. A Elhabazi, et al. (2001) J Immunol. 166(7):4341-7. 4. John R Basile, et al. (2007) J Biol Chem. 282(9):6899-905. 5. Jose Rafael Sierra, et al. (2008) J Exp Med. 205(7):1673-85.
