Recombinant Human RNASE1 protein (rFc Tag) (HPLC verified)
种属
Human
纯度
>90 %, SDS-PAGE
>90 %, SEC-HPLC
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human RNASE1 protein Lys29-Thr156 (Accession# P07998) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 6035 |
| 蛋白编号 | P07998 |
| 预测分子量 | 40.6 kDa |
| SDS-PAGE | 42-55 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Ribonucleases (RNases) are enzymes that catalyze the degradation of RNA into smaller components. They play crucial roles in various biological processes, including RNA maturation, immune defense, and cellular stress responses. In humans, several types of RNases have been identified and characterized. The ribonuclease A (RNase A) family is one of the best-characterized vertebrate-specific proteins, and in humans, eight catalytically active RNases (numbered 1–8) have been identified and have unique tissue distributions. Apart from the digestion of dietary RNA, these RNases have a broad range of biological actions, including the regulation of intra- or extra-cellular RNA metabolism, antiviral, antibacterial, and antifungal activities, neurotoxicity, promotion of cell proliferation, anti-apoptosis, and immunomodulatory abilities. Based on multiple biological roles, RNases are found to participate in the pathogenic processes of many diseases, such as infection, immune dysfunction, neurodegeneration, cancer, and cardiovascular disorders.
参考文献:
1. Rosenberg HF. (2008) J Leukoc Biol. 83(5):1079-87. 2. Michlewski G, (2010) RNase-assisted RNA chromatography. 16(8):1673-8. 3. Bao L, Hu J, et al. (2023) Nat Commun. 14(1):2280. 4. Rudler DL, Siira SJ, et al. (2023) Methods Mol Biol. 2661:317-328.
