Recombinant Human PAPPA2 protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human PAPPA2 protein Ser234-Cys1396 (Accession# Q9BXP8-1) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 60676 |
| 蛋白编号 | Q9BXP8-1 |
| 预测分子量 | 155.2 kDa |
| SDS-PAGE | 140-220 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
PAPPA2 (pregnancy-associated plasma protein-A2) was discovered as a protease which specifically cleaves IGF-binding protein (IGFBP)-3 and -5. PAPPA2 is very highly expressed in the placenta relative to all other tissues. PAPPA2 mRNA and protein are increased in association with pre-eclampsia. PAPPA2 deficiency results in growth impairment in humans with expected derangements in biochemical parameters including increased levels of total IGF-1, IGFBP-3, ALS, and growth hormone.
参考文献:
1. Sifakis S, et al. (2018). Mol Med Rep. May;17(5):6435-6440. 2. Fujimoto M, et al. (2020). Mol Cell Endocrinol. Dec 1;518:110967. 3. Andrew M, et al. (2018). J Endocr Soc. May 28;2(7):646-656.
