Recombinant Human Osteopontin protein (hFc Tag)
验证数据展示
产品信息
| 纯度 | >80 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Immobilized Human ITGAV (GST tag) at 1 μg/mL (100 μL/well) can bind Human Osteopontin (hFc tag) with a linear range of 0.14 -0.56 μg/mL. |
| 来源 | HEK293-derived Human Osteopontin protein Ile17-Asn300 (Accession# P10451-5) with a human IgG1 Fc tag at the C-terminus. |
| 基因ID | 6696 |
| 蛋白编号 | P10451-5 |
| 预测分子量 | 59.5 kDa |
| SDS-PAGE | 85-95 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone. OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues. It can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.
参考文献:
1. Denhardt DT, et al. (2001). J Clin Invest. 107(9):1055-61. 2. Fisher LW, et al. (2001). Biochem Biophys Res Commun. 280(2):460-5. 3. Rittling SR, et al. (2004). Br J Cancer. 90(10):1877-81. 4. Rangaswami H, et al. (2006). Trends Cell Biol. 16(2):79-87.
Publications
| Species | Title |
|---|---|
Sci Rep IL-1β alleviates suppressive potential of bone marrow mesenchymal stem cells in macrophage M1 polarization via autophagy mediated degradation of SPP1. |
