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Recombinant human IGF-I/IGF-1 protein

种属

Human

纯度

>90 %, SDS-PAGE

标签

hFc Tag

生物活性

EC50: 20-80 ng/mL

Cat no : Eg0206

Print datasheet

Synonyms

IGF-1, IGF I, IGFI, IGF-I, Insulin like growth factor I

验证数据展示

  • Purity of Recombinant Human IGF1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IGF1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human IGF1 R (GST tag) at 1 μg/mL (100 μL/well) can bind Human IGF1 (hFc tag) with a linear range of 20-80 ng/mL.

    Immobilized Human IGF1 R (GST tag) at 1 μg/mL (100 μL/well) can bind Human IGF1 (hFc tag) with a linear range of 20-80 ng/mL.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Immobilized Human IGF1 R (GST tag) at 1 μg/mL (100 μL/well) can bind Human IGF1 (hFc tag) with a linear range of 20-80 ng/mL.
来源 HEK293-derived Human IGF1 protein Gly49-Ala118 (Accession# P05019-1) with a human IgG1 Fc tag at the N-terminus.
基因ID 3479
蛋白编号 P05019-1
预测分子量 33.7 kDa
SDS-PAGE 34-36 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone is the principal mediator of biochemical effects of growth hormone (GH). IGF1 is an important growth factor in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 have been shown to play an essential role in preventing the formation of fatty liver. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas.

参考文献:

1. Adamo ML. et al. (1993) Adv Exp Med Biol. 343:1-11.
2. Adachi Y. et al. (2019) J Gastroenterol Hepatol. 2019 Jun 3.
3. Anisimov VN. et al. (2019) Crit Rev Oncol Hematol. 87(3):201-23.
4. Sonntag WE. et al. (2012) J Gerontol A Biol Sci Med Sci. 67(6):587-98.
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