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Recombinant Human CD53 protein (rFc Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

rFc Tag

生物活性

未测试

Cat no : Eg2182

Print datasheet

Synonyms

Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin 25, Tetraspanin-25

验证数据展示

  • Purity of Recombinant Human CD53 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD53 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human CD53 protein Glu107-Asn181 (Accession# P19397) with a rabbit IgG Fc tag at the C-terminus.
基因ID 963
蛋白编号 P19397
预测分子量 34.6 kDa
SDS-PAGE 38-50 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CD53 is a structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. It participates in diverse biological functions such as cell signal transduction, adhesion, migration, and protein trafficking. It also plays a role in the activation of monocytes and B-cells.

参考文献:

1. Malou Zuidscherwoude, et al. (2017) Sci Signal. May 9;10(478):eaag2755. 
2. Yihu Yang, et al. (2020) EMBO J. Sep 15;39(18):e105246.
3. Vera-Marie E Dunlock, Abbey B Arp, et al. (2022) Cell Rep. Jun 28;39(13):111006.
4. J Olweus, F Lund-Johansen, V Horejsi. (1993) J Immunol. Jul 15;151(2):707-16.