ChromoTek Histone-Chromobody® plasmid (eGFP)

DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.

Specificity

Histone

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

EGFP

Cat No : tcg

Synonyms

Chromobody, Histone Chromobody



Product Information

DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.

Description The ChromoTek Histone-Chromobody* is a probe to visualize chromatin dynamics in live cells.

• Easily transfect your cells with the Histone-Chromobody plasmid

• Histone-Chromobody as marker of chromatin, nucleosomes, and histones

• Histone-Chromobody does not intercalate in DNA

• Histone-Chromobody is non-toxic for cells over extended periods of time (up to 3 days)

*Commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment
ApplicationsNon-invasive live cell imaging
Monitors chromatin dynamics
Histone and chromatin as biomarker
SpecificityHistone H2A-H2B heterodimer, tested in mammalian cells, flies, and yeast
ReporterEGFP
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SelectionKan/Neo
SequenceWith the Histone-Chromobody plasmid you receive the sequence information of the alpaca antibody to H2A-H2B heterodimer fused to EGFP and the full vector sequence.
Transfection Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Nat Commun

Helicase LSH/Hells regulates kinetochore function, histone H3/Thr3 phosphorylation and centromere transcription during oocyte meiosis.

Authors - Claudia Baumann