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验证数据展示

  • WB analysis using 86716-1-RR (same clone as 86716-1-PBS)

    WB analysis using 86716-1-RR (same clone as 86716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86716-1-RR (Glutamine synthetase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86716-1-PBS in a different storage buffer formulation.

  • WB analysis using 86716-1-RR (same clone as 86716-1-PBS)

    WB analysis using 86716-1-RR (same clone as 86716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86716-1-RR (Glutamine synthetase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86716-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 86716-1-RR (same clone as 86716-1-PBS)

    IP experiment of mouse liver using 86716-1-RR (same clone as 86716-1-PBS)

    IP result of anti-Glutamine synthetase (IP:86716-1-RR, 4ug; Detection:86716-1-RR 1:2000) with mouse liver tissue lysate 2480 ug. This data was developed using the same antibody clone with 86716-1-PBS in a different storage buffer formulation.

Glutamine synthetase Recombinant monoclonal antibody, PBS Only

Glutamine synthetase Uni-rAb® Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 86716-1-PBS

产品说明书

CloneNo. 251711A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

GLUL, EC:2.3.1.225, EC:6.3.1.2, GLNS, Glutamate--ammonia ligase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.

产品信息

86716-1-PBS targets Glutamine synthetase in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1510

Product name: Recombinant human Glutamine synthetase protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-373 aa of BC011700

Sequence: MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutamate-ammonia ligase (glutamine synthetase)
别名 GLUL, EC:2.3.1.225, EC:6.3.1.2, GLNS, Glutamate--ammonia ligase
计算分子量 374 aa, 42 kDa
观测分子量 40-42 kDa
GenBank蛋白编号BC011700
基因名称 Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_3745075
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15104
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137).

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