Validation Data Gallery
Product Information
Alpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin. Compatible with avidin, streptavidin and neutravidin reagents.
| Description | Alpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin for extraordinary stable & reliable binding
• GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate • Fulfills highest requirements for antibody validation • Structure and function are characterized |
| Applications | ELISA Surface Plasmon Resonance (SPR) coating of multiwell plates |
| Specificity/Target | CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine For the complete list, please click here: Fluorescent protein specificity table |
| Conjugate | Biotin (PEG4-Biotin) |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 1 pM |
| RRID | AB_2827557 |
| Storage Buffer | Buffered aqueous solution (25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA), Preservative: 0.09% Sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at +4°C / 40°F. Stable for one year. Do not freeze. |
| Size | 250 µg |
Publications
| Application | Title |
|---|---|
Science Evolutionary-scale enzymology enables exploration of a rugged catalytic landscape | |
Nat Nanotechnol Oligomeric organization of membrane proteins from native membranes at nanoscale spatial and single-molecule resolution | |
Nat Methods A proteome-wide quantitative platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs | |
Nat Commun Automating data analysis for hydrogen/deuterium exchange mass spectrometry using data-independent acquisition methodology | |
Nat Commun Targeting the actin nucleation promoting factor WASp provides a therapeutic approach for hematopoietic malignancies. |
