验证数据展示
经过测试的应用
| Positive WB detected in | HaCaT cells, U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16415-1-AP targets GALNT12 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag9571 Product name: Recombinant human GALNT12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC013945 Sequence: MAGGLFAVSKKYFEYLGSYDTGMEVWGGENLEFSFRIWQCGGVLETHPCSHVGHVFPKQAPYSRNKALANSVRAAEVWMDEFKELYYHRNPRARLEPFGDVTERKQLRDKLQCKDFKWFLETVYPELHVPEDRPGFFGMLQNKGLTDYCFDYNPPDENQIVGHQVILYLCHGMGQNQFFEYTSQKEIRYNTHQPEGCIAVEAGMDTLIMHLCEETAPENQKFILQEDGSLFHEQSKKCVQAARKESSDSFVPLLRDCTNSDHQKWFFKERML 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 (GalNAc-T12) |
| 别名 | EC:2.4.1.41, GalNAc T12, GalNAc-T12, Polypeptide N-acetylgalactosaminyltransferase 12, pp GaNTase 12 |
| 计算分子量 | 581 aa, 67 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC013945 |
| 基因名称 | GALNT12 |
| Gene ID (NCBI) | 79695 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IXK2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Polypeptide N‑acetylgalactosaminyl transferase 12 (GALNT12) belongs to the uridine diphosphate N‑acetylgalactosamine gene family, which is involved in numerous biological processes of diseases, such as tumor progression. GALNT12 is highly expressed in the digestive tract and it is frequently downregulated in colorectal cancer (CRC) (PMID: 29095867). Genetic knockdown (KD) and knockout (KO) of GALNT12 in U87 MG, a representative GBM cell line with high GALNT12 expression, confirmed that GALNT12 deficiency leads to decreased cell proliferation, migration and invasion (PMID: 35461073).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GALNT12 antibody 16415-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
