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FNTB Monoclonal antibody

FNTB Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F3B10

Cat No : 66783-1-Ig

Print datasheet

Synonyms

FNTB, FPTB, FTase beta

验证数据展示

  • WB analysis using 66783-1-Ig

    WB analysis using 66783-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66783-1-Ig

    WB analysis of HeLa using 66783-1-Ig

    WB result of FNTB antibody (66783-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected HeLa cells.

  • IF Staining of A431 using 66783-1-Ig

    IF Staining of A431 using 66783-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FNTB antibody (66783-1-Ig, Clone: 1F3B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells, 4T1 cells
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66783-1-Ig targets FNTB in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen FNTB fusion protein Ag27796 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name farnesyltransferase, CAAX box, beta
Synonyms FNTB, FPTB, FTase beta
Calculated Molecular Weight 437 aa, 49 kDa
Observed Molecular Weight 44-45 kDa
GenBank Accession NumberBC020232
Gene Symbol FNTB
Gene ID (NCBI) 2342
RRIDAB_2882128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49356
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.

实验方案

Product Specific Protocols
WB protocol for FNTB antibody 66783-1-IgDownload protocol
IF protocol for FNTB antibody 66783-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols