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验证数据展示

  • WB analysis of MCF-7 using 12842-1-AP (same clone as 12842-1-PBS)

    WB analysis of MCF-7 using 12842-1-AP (same clone as 12842-1-PBS)

    WB result of FBP1 antibody (12842-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBP1 transfected MCF-7 cells. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

  • WB analysis using 12842-1-AP (same clone as 12842-1-PBS)

    WB analysis using 12842-1-AP (same clone as 12842-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12842-1-AP (FBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 12842-1-AP (same clone as 12842-1-PBS)

    IP experiment of mouse liver using 12842-1-AP (same clone as 12842-1-PBS)

    IP result of anti-FBP1 (IP:12842-1-AP, 4ug; Detection:12842-1-AP 1:10000) with mouse liver tissue lysate 2480 ug. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 12842-1-AP (same clone as 12842-1-PBS)

    IP experiment of MCF-7 using 12842-1-AP (same clone as 12842-1-PBS)

    IP result of anti-FBP1 (IP:12842-1-AP, 4ug; Detection:12842-1-AP 1:10000) with MCF-7 cells lysate 1200 ug. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12842-1-AP (same clone as 12842-1-PBS)

    IHC staining of human kidney using 12842-1-AP (same clone as 12842-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBP1 antibody included
  • IHC staining of human kidney using 12842-1-AP (same clone as 12842-1-PBS)

    IHC staining of human kidney using 12842-1-AP (same clone as 12842-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBP1 antibody included
  • Featured Product
  • KD/KO Validated

FBP1 Polyclonal antibody, PBS Only

FBP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12842-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

D-fructose-1,6-bisphosphate 1-phosphohydrolase 1, EC:3.1.3.11, FBP, FBPase 1, Fructose-1,6-bisphosphatase 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FBP1 Ready-To-Use IHC Kit. FBP1 primary antibody included.

产品信息

12842-1-PBS targets FBP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3837

Product name: Recombinant human FBP1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-338 aa of BC012927

Sequence: MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fructose-1,6-bisphosphatase 1
别名 D-fructose-1,6-bisphosphate 1-phosphohydrolase 1, EC:3.1.3.11, FBP, FBPase 1, Fructose-1,6-bisphosphatase 1
计算分子量 338 aa, 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC012927
基因名称 FBP1
Gene ID (NCBI) 2203
RRIDAB_2103572
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09467
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).

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