验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32520-1-AP targets FANCE in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38546 Product name: Recombinant human FANCE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 274-535 aa of NM_021922.2 Sequence: LAESLELPKAIQDQLPRLQQLLKTLEEGLEGLEDAPPVELQLLHECSPSQMDLLCAQLQLPQLSDLGLLRLCTWLLALSPDLSLSNATVLTRSLFLGRILSLTSSASRLLTTALTSFCAKYTYPVCSALLDPVLQAPGTGPAQTELLCCLVKMESLEPDAQVLMLGQILELPWKEETFLVLQSLLERQVEMTPEKFSVLMEKLCKKGLAATTSMAYAKLMLTVMTKYQANITETQRLGLAMALEPNTTFLRKSLKAALKHLGP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Fanconi anemia, complementation group E |
| 别名 | FACE, Fanconi anemia group E protein, Protein FACE |
| 计算分子量 | 59 kDa，536aa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | NM_021922.2 |
| 基因名称 | FANCE |
| Gene ID (NCBI) | 2178 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9HB96 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fanconi anemia group E protein (FANCE) is primarily localized in the nucleoplasm, with additional distribution in mitotic chromosomes and centrosomes. As a key component of the Fanconi anemia (FA) core complex, it plays an essential role in DNA interstrand cross-link repair, serving as a critical bridge between the FA complex and FANCD2 protein (PMID: 12239156; 12093742; 17308347). Cells with FANCE defects exhibit hypersensitivity to DNA cross-linking agents, chromosomal instability, and impaired DNA repair capacity (PMID: 21948210; 28239445; 37563658).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FANCE antibody 32520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
