验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, mouse brain tissue, mouse cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67044-1-Ig targets EPAC2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag5186 Product name: Recombinant human RAPGEF4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 712-1011 aa of BC024004 Sequence: VKMSSGGEKVVLKPNDVSVFMTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Rap guanine nucleotide exchange factor (GEF) 4 |
| 别名 | CAMP GEFII, CGEF2, EPAC 2, EPAC2, Nbla00496, RAPGEF4 |
| 计算分子量 | 116 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC024004 |
| 基因名称 | EPAC2/RAPGEF4 |
| Gene ID (NCBI) | 11069 |
| RRID | AB_2882358 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8WZA2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPAC2 antibody 67044-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
