验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue, L02 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DPYD Ready-To-Use IHC Kit. DPYD primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27662-1-AP targets DPYD in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag26599 Product name: Recombinant human DPYD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-99 aa of BC008379 Sequence: VLSKDSADIESILALNPRTQTHATLCSTSAKKLDKKHWKRNPDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dihydropyrimidine dehydrogenase |
| 别名 | DHP, DHPDHASE, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPD, DPYD |
| 计算分子量 | 1025 aa, 111 kDa |
| 观测分子量 | 111 kDa |
| GenBank蛋白编号 | BC008379 |
| 基因名称 | DPYD |
| Gene ID (NCBI) | 1806 |
| RRID | AB_2880941 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12882 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DPYD antibody 27662-1-AP | Download protocol |
| WB protocol for DPYD antibody 27662-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Pharmgenomics Pers Med Exploration and Validation of Pancreatic Cancer Hub Genes Based on Weighted Gene Co-Expression Network Analysis and Immune Infiltration Score Analysis | ||
J Cell Mol Med Pathway-based stratification of gliomas uncovers four subtypes with different TME characteristics and prognosis | ||
Theranostics Single cell analyses reveal the PD-1 blockade response-related immune features in hepatocellular carcinoma | ||
J Clin Invest CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes | ||
Nat Commun Proteomic profiling of gliomas unveils immune and metabolism-driven subtypes with implications for anti-nucleotide metabolism therapy | ||
Clin Transl Oncol Pyrimidine metabolism reshapes immune microenvironment and implies poor prognosis in glioma
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