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验证数据展示

  • WB analysis of A431 using 25162-1-AP

    WB analysis of A431 using 25162-1-AP

    WB result of DNAJC18 antibody (25162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC18 transfected A431 cells.

  • WB analysis using 25162-1-AP

    WB analysis using 25162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25162-1-AP (DNAJC18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25162-1-AP

    IHC staining of mouse testis using 25162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25162-1-AP (DNAJC18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25162-1-AP

    IHC staining of mouse testis using 25162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25162-1-AP (DNAJC18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 25162-1-AP

    IF Staining of U2OS using 25162-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC18 antibody (25162-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 25162-1-AP

    WB analysis using 25162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25162-1-AP (DNAJC18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

DNAJC18 Polyclonal antibody

DNAJC18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25162-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

DNAJC 18

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25162-1-AP targets DNAJC18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18498

Product name: Recombinant human DNAJC18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 196-358 aa of BC030162

Sequence: TDDTYYYRRRHRHERTQTQKEEEEEKPQTTYSAFIQLLPVLVIVIISVITQLLATNPPYSLFYKSTLGYTISRETQNLQVPYFVDKNFDKAYRGASLHDLEKTIEKDYIDYIQTSCWKEKQQKSELTNLAGLYRDERLKQKAESLKLENCEKLSKLIGLRRGG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 18
别名 DNAJC 18
计算分子量 358 aa, 42 kDa
观测分子量 39-42 kDa
GenBank蛋白编号BC030162
基因名称 DNAJC18
Gene ID (NCBI) 202052
RRIDAB_2918087
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H819
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DnaJC18, also named as DnaJ (Hsp40) homolog, subfamily C, member 21, belongs to Type III DnaJ Family. Type III DnaJ proteins have a J domain but lack the other sequence features that are found in type I and II members of the family. DnaJC18 might play a role in the process of spermatogenesis in adult testis (PMID: 29082339).

实验方案

Product Specific Protocols
IF protocol for DNAJC18 antibody 25162-1-APDownload protocol
IHC protocol for DNAJC18 antibody 25162-1-APDownload protocol
WB protocol for DNAJC18 antibody 25162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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