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验证数据展示

  • WB analysis of fetal human brain using 84595-4-RR (same clone as 84595-4-PBS)

    WB analysis of fetal human brain using 84595-4-RR (same clone as 84595-4-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 84595-4-RR (same clone as 84595-4-PBS)

    WB analysis of SH-SY5Y using 84595-4-RR (same clone as 84595-4-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 84595-4-RR (same clone as 84595-4-PBS)

    IHC staining of rat brain using 84595-4-RR (same clone as 84595-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84595-4-RR (DCX antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84595-4-RR

    Affinity and Kinetic Characterization of 84595-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84595-4-RR against Human DCX were performed. The affinity constant is 1.23 nM.

DCX Recombinant monoclonal antibody, PBS Only

DCX Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84595-4-PBS

产品说明书

CloneNo. 241985H1

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84595-4-PBS targets DCX in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag4945

Product name: Recombinant human DCX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-360 aa of BC027925

Sequence: MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 doublecortin
别名 Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1
计算分子量 402 aa, 45 kDa
观测分子量 40 kDa
GenBank蛋白编号BC027925
基因名称 DCX
Gene ID (NCBI) 1641
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO43602
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

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