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验证数据展示

  • WB analysis using 85047-5-RR

    WB analysis using 85047-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85047-5-RR (DCP1A antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85047-5-RR

    IF Staining of HepG2 using 85047-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (85047-5-RR, Clone: 242553F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

DCP1A Recombinant antibody

DCP1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85047-5-RR

产品说明书

CloneNo. 242553F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, Jurkat cells, MCF-7 cells, SW480 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DCP1A Ready-To-Use IHC Kit. DCP1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85047-5-RR targets DCP1A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DCP1A fusion protein Ag18027 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DCP1 decapping enzyme homolog A (S. cerevisiae)
别名 EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360
计算分子量 582 aa, 63 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC007439
基因名称 DCP1A
Gene ID (NCBI) 55802
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

实验方案

Product Specific Protocols
WB protocol for DCP1A antibody 85047-5-RRDownload protocol
IF protocol for DCP1A antibody 85047-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols