DAD1 Polyclonal antibody
DAD1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10531-1-AP targets DAD1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
Tested Reactivity | human |
Immunogen | DAD1 fusion protein Ag0814 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | defender against cell death 1 |
Synonyms | DAD 1, DAD1, defender against cell death 1, OST2 |
Calculated Molecular Weight | 12 kDa |
Observed Molecular Weight | 16-20 kDa |
GenBank Accession Number | BC007403 |
Gene Symbol | DAD1 |
Gene ID (NCBI) | 1603 |
RRID | AB_2089878 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P61803 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DAD1 antibody 10531-1-AP | Download protocol |
IHC protocol for DAD1 antibody 10531-1-AP | Download protocol |
IF protocol for DAD1 antibody 10531-1-AP | Download protocol |
IP protocol for DAD1 antibody 10531-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |