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验证数据展示

  • WB analysis using 86313-1-RR (same clone as 86313-1-PBS)

    WB analysis using 86313-1-RR (same clone as 86313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86313-1-RR (Cytokeratin 80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skin using 86313-1-RR (same clone as 86313-1-PBS)

    IF Staining of mouse skin using 86313-1-RR (same clone as 86313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skin using 86313-1-RR (same clone as 86313-1-PBS)

    IF Staining of mouse skin using 86313-1-RR (same clone as 86313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

Cytokeratin 80 Recombinant monoclonal antibody, PBS Only

Cytokeratin 80 Uni-rAb® Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 86313-1-PBS

产品说明书

CloneNo. 250883H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, Indirect ELISA

别名

KRT80, CK 80, CK-80, Cytokeratin-80, K80

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86313-1-PBS targets Cytokeratin 80 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 80
别名 KRT80, CK 80, CK-80, Cytokeratin-80, K80
计算分子量 422 aa, 47 kDa
观测分子量50 kDa
GenBank蛋白编号BC065180
基因名称 KRT80
Gene ID (NCBI) 144501
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6KB66
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.

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