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验证数据展示

  • WB analysis using 86153-7-RR (same clone as 86153-7-PBS)

    WB analysis using 86153-7-RR (same clone as 86153-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86153-7-RR (CK7-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

    IHC staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IF Staining of HEPG2 using 86153-7-RR (same clone as 86153-7-PBS)

    IF Staining of HEPG2 using 86153-7-RR (same clone as 86153-7-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEPG2 cells using Cytokeratin 7 antibody (86153-7-RR, Clone: 242229F1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

    IHC staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IF Staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

    IF Staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 7 antibody (86153-7-RR, Clone: 242229F1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

    IF Staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 7 antibody (86153-7-RR, Clone: 242229F1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

Cytokeratin 7 Recombinant monoclonal antibody, PBS Only

Cytokeratin 7 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 86153-7-PBS

产品说明书

CloneNo. 242229F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

KRT7, CK 7, CK7, CK-7, Cytokeratin,Keratin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.

产品信息

86153-7-PBS targets Cytokeratin 7 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 7
别名 KRT7, CK 7, CK7, CK-7, Cytokeratin,Keratin
计算分子量 51 kDa
观测分子量51 kDa
GenBank蛋白编号NM_005556
基因名称 Cytokeratin 7
Gene ID (NCBI) 3855
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08729
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytokeratin 7 (CK7) is a type II keratin protein that is a principal constituent of the intermediate filament cytoskeleton. It is primarily expressed in simple epithelia lining the cavities of internal organs, glandular ducts, and blood vessels. Abnormal expression of CK7 has been linked to various pathological conditions, including cancer. Overexpression of CK7 promotes tumor progression and metastasis in different human cancers, and its suppression leads to rapid tumor regression, highlighting its potential as a therapeutic target CK7 is also involved in inhibiting interferon-dependent interphase, promoting DNA synthesis, initiating translation possibly through interaction with p150 (the largest subunit of eukaryotic translation initiation factor 3), and interacting with G protein-coupled estrogen receptor 1 (GPER1), which activates several signaling pathways. In the context of cancer diagnosis, CK7 is used as a marker to distinguish between different types of carcinomas. It is expressed in most adenocarcinomas, particularly those of the lung and colorectal origin, and is useful in differentiating primary ovarian carcinoma from metastatic colorectal carcinoma. CK7 expression has also been studied as a predictor of an unfavorable prognosis in colorectal carcinoma.

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