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验证数据展示

  • WB analysis using 86277-2-RR (same clone as 86277-2-PBS)

    WB analysis using 86277-2-RR (same clone as 86277-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86277-2-RR (KRT39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse esophagus using 86277-2-RR (same clone as 86277-2-PBS)

    IHC staining of mouse esophagus using 86277-2-RR (same clone as 86277-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse esophagus using 86277-2-RR (same clone as 86277-2-PBS)

    IHC staining of mouse esophagus using 86277-2-RR (same clone as 86277-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse skin using 86277-2-RR (same clone as 86277-2-PBS)

    IF Staining of mouse skin using 86277-2-RR (same clone as 86277-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 39 antibody (86277-2-RR, Clone: 250891F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 86277-2-RR (same clone as 86277-2-PBS)

    IF Staining of MCF-7 using 86277-2-RR (same clone as 86277-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 39 antibody (86277-2-RR, Clone: 250891F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

Cytokeratin 39 Recombinant monoclonal antibody, PBS Only

Cytokeratin 39 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 86277-2-PBS

产品说明书

CloneNo. 250891F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

CK-39, Cytokeratin-39, K39, KA35, Keratin, type I cytoskeletal 39

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86277-2-PBS targets Cytokeratin 39 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 39
别名 CK-39, Cytokeratin-39, K39, KA35, Keratin, type I cytoskeletal 39
观测分子量45 kDa
GenBank蛋白编号BC157887
基因名称 KRT39
Gene ID (NCBI) 390792
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6A163
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytokeratin 39 (CK-39) is a member of the cytokeratin family, a group of intermediate filament proteins primarily expressed in epithelial cells. Cytokeratins are classified into Type I (acidic) and Type II (basic) subfamilies, which pair to form heteropolymers essential for maintaining cellular structural integrity, mechanical stability, and stress resistance. CK-39 is predominantly expressed in stratified and glandular epithelia, such as those found in the prostate, bladder, and respiratory tract. Its expression pattern may vary depending on tissue differentiation states.

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