验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy KRT16 Ready-To-Use IHC Kit. KRT16 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86235-1-RR targets Cytokeratin 16 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | keratin 16 |
| 别名 | KRT16, CK 16, CK16, CK-16, Cytokeratin,Keratin |
| 计算分子量 | 473 aa, 51 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC039169 |
| 基因名称 | Cytokeratin 16 |
| Gene ID (NCBI) | 3868 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08779 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytokeratin 16 is a Type I acidic keratin (classified as KRT16 in the human gene nomenclature) that pairs with Type II basic keratins (e.g., CK-6) to form intermediate filaments in epithelial cells. It is prominently expressed in stratified epithelia, particularly under conditions of stress, proliferation, or pathology. CK-16 is constitutively expressed in specialized epithelia (e.g., nail beds, hair follicles, oral mucosa) but is normally absent in healthy, quiescent skin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 16 antibody 86235-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
