验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, human milk, A549 cells |
| Positive IHC detected in | human tonsillitis tissue, human cervical cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66802-1-Ig targets Cytokeratin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag11240 Product name: Recombinant human KRT16 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 121-473 aa of BC039169 Sequence: MQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | keratin 16 |
| 别名 | KRT16, 2H4D8, CK 16, CK16, CK-16 |
| 计算分子量 | 473 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC039169 |
| 基因名称 | Cytokeratin 16 |
| Gene ID (NCBI) | 3868 |
| RRID | AB_2882145 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P08779 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
| WB protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancers (Basel) Emerging Insights into Keratin 16 Expression during Metastatic Progression of Breast Cancer.
| ||
Nat Commun Spatial transcriptomics analysis of esophageal squamous precancerous lesions and their progression to esophageal cancer | ||
Genomics Identification of prognostic biomarkers for cholangiocarcinoma by combined analysis of molecular characteristics of clinical MVI subtypes and molecular subtypes
| ||
J Photochem Photobiol B Acne-related UVA-induced facial fluorescence: An exploratory study from physiological properties to tissue structure information |
