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验证数据展示

  • WB analysis using 82824-1-RR (same clone as 82824-1-PBS)

    WB analysis using 82824-1-RR (same clone as 82824-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 82824-1-RR (same clone as 82824-1-PBS)

    IHC staining of human oesophagus cancer using 82824-1-RR (same clone as 82824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT14 antibody included
  • IHC staining of human oesophagus cancer using 82824-1-RR (same clone as 82824-1-PBS)

    IHC staining of human oesophagus cancer using 82824-1-RR (same clone as 82824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT14 antibody included
  • WB analysis of A431 using 82824-1-RR (same clone as 82824-1-PBS)

    WB analysis of A431 using 82824-1-RR (same clone as 82824-1-PBS)

    WB result of Cytokeratin 14 antibody (82824-1-RR; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 14 transfected A431 cells. This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

  • IHC staining of Human bowens disease using 82824-1-RR (same clone as 82824-1-PBS)

    IHC staining of Human bowens disease using 82824-1-RR (same clone as 82824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT14 antibody included
  • IHC staining of Human bowens disease using 82824-1-RR (same clone as 82824-1-PBS)

    IHC staining of Human bowens disease using 82824-1-RR (same clone as 82824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 82824-1-RR (Cytokeratin 14 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT14 antibody included
  • IF Staining of A431 using 82824-1-RR (same clone as 82824-1-PBS)

    IF Staining of A431 using 82824-1-RR (same clone as 82824-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 14 antibody (82824-1-RR, Clone: 1L19 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82824-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82824-1-RR

    Affinity and Kinetic Characterization of 82824-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82824-1-RR against Human Cytokeratin 14 were performed. The affinity constant is 0.989 nM.

  • Featured Product
  • KD/KO验证

Cytokeratin 14 Recombinant antibody, PBS Only

Cytokeratin 14 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82824-1-PBS

产品说明书

CloneNo. 1L19

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KRT14 Ready-To-Use IHC Kit. KRT14 primary antibody included.

产品信息

82824-1-PBS targets Cytokeratin 14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 14 fusion protein Ag17559 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 14
别名 KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14
计算分子量 472 aa, 52 kDa
观测分子量52 kDa
GenBank蛋白编号BC002690
基因名称 Cytokeratin 14
Gene ID (NCBI) 3861
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02533
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytokeratin 14, one of about 20 different cytokeratin isotypes of human cells, is the intermediate filament protein characteristic of epithelial cells. Cytokeratin 14 is expressed in the basal compartment of all stratified squamous epithelia. In various kinds of human tumors, the appearance and increasing expression of Cytokeratin 14 were strikingly associated with higher grade and stage of carcinoma, with varying degrees of unfavorable prognosis. In lung squamous cell carcinoma(LSCC), Cytokeratin 14 was expressed in the tumor cell nests showing stromal invasion with fibrosis and lymph node metastases, indicating that Cytokeratin 14 involved in proliferation and metastasis of LSCC. Cytokeratin 14 expression is sometimes used in diagnosis of myoepithelioma1 and intraductal vs. invasive ductal carcinoma of the breast.