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验证数据展示

  • WB analysis using 12278-1-AP (same clone as 12278-1-PBS)

    WB analysis using 12278-1-AP (same clone as 12278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)

    IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)

    IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IP experiment of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)

    IP experiment of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)

    IP result of anti-Calretinin (IP:12278-1-AP, 4ug; Detection:12278-1-AP 1:10000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

Calretinin Polyclonal antibody, PBS Only

Calretinin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12278-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

CALB2, 29 kDa calbindin, CAB29, CAL2, calbindin 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.

产品信息

12278-1-PBS targets Calretinin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2924

Product name: Recombinant human Calretinin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-271 aa of BC015484

Sequence: MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSTEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLYRKDLEIVLCSEPPM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calbindin 2
别名 CALB2, 29 kDa calbindin, CAB29, CAL2, calbindin 2
计算分子量 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC015484
基因名称 Calretinin
Gene ID (NCBI) 794
RRIDAB_2228338
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22676
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

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