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验证数据展示

  • WB analysis using 84427-4-RR

    WB analysis using 84427-4-RR

    IFN gamma and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 84427-4-RR (CXCL9 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours.

CXCL9/MIG Recombinant antibody

CXCL9/MIG Recombinant Antibody for WB, ELISA
Cat No. 84427-4-RR

产品说明书

CloneNo. 241437F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

MIG; CXCL9, MIG, CXCL9, C-X-C motif chemokine 9, CMK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLPS and IFN gamma treated THP-1 cells, IFN gamma treated THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84427-4-RR targets CXCL9/MIG in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CXCL9/MIG fusion protein Ag17932 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X-C motif) ligand 9
别名 MIG; CXCL9, MIG, CXCL9, C-X-C motif chemokine 9, CMK
计算分子量 14 kDa
观测分子量14 kDa
GenBank蛋白编号BC095396
基因名称 CXCL9
Gene ID (NCBI) 4283
RRIDAB_3671954
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ07325
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCL9 is a small cytokine known as MIG. CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. A high level of CXCL9 revealed in peripheral liquids, can be considered as a marker of host immune response, especially of that involving Th1 cells.

实验方案

Product Specific Protocols
WB protocol for CXCL9/MIG antibody 84427-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols