验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83937-3-RR targets CXCL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | CXCL1 fusion protein Ag2917 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) |
| 别名 | GRO-alpha(5-73), GRO-alpha(4-73), CXCL 1, C-X-C motif chemokine 1, C X C motif chemokine 1 |
| 计算分子量 | 8 kDa to 14 kDa |
| 观测分子量 | 11 kDa |
| GenBank蛋白编号 | BC011976 |
| 基因名称 | CXCL1 |
| Gene ID (NCBI) | 2919 |
| RRID | AB_3671519 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P09341 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CXCL1 antibody 83937-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |