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验证数据展示

  • WB analysis using 83738-5-RR

    WB analysis using 83738-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83738-5-RR (Cx3cl1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83738-5-RR

    IHC staining of mouse brain using 83738-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83738-5-RR (CX3CL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83738-5-RR

    IHC staining of mouse brain using 83738-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83738-5-RR (CX3CL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83738-5-RR

    Affinity and Kinetic Characterization of 83738-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83738-5-RR against Mouse CX3CL1 were performed. The affinity constant is 0.744 nM.

CX3CL1 Recombinant monoclonal antibody

CX3CL1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83738-5-RR

产品说明书

CloneNo. 240738E2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Scyd1, Cxc3, CX3C, ABCD 3, 240738E2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse lung tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83738-5-RR targets CX3CL1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X3-C motif) ligand 1
别名 Scyd1, Cxc3, CX3C, ABCD 3, 240738E2
计算分子量42 kDa
观测分子量55-75 kDa
GenBank蛋白编号NM_009142.3
基因名称 Cx3cl1
Gene ID (NCBI) 20312
RRIDAB_3671337
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO35188
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CX3CL1, also known as fractalkine, is a trans-membrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1 (PMID: 9479488; 23125415). CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation (PMID: 21045240). CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage (PMID: 11495925; 12714508).

实验方案

Product Specific Protocols
IHC protocol for CX3CL1 antibody 83738-5-RRDownload protocol
WB protocol for CX3CL1 antibody 83738-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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