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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • IP experiment of HeLa using 83619-5-RR (same clone as 83619-5-PBS)

    IP experiment of HeLa using 83619-5-RR (same clone as 83619-5-PBS)

    IP result of anti-CUL3 (IP:83619-5-RR, 4ug; Detection:83619-5-RR 1:4000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 83619-5-PBS in a different storage buffer formulation.

  • WB analysis using 83619-5-RR (same clone as 83619-5-PBS)

    WB analysis using 83619-5-RR (same clone as 83619-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83619-5-RR (CUL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83619-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83619-5-RR

    Affinity and Kinetic Characterization of 83619-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83619-5-RR against Human CUL3 were performed. The affinity constant is below 1 pM.

CUL3 Recombinant antibody, PBS Only

CUL3 Recombinant Antibody for WB, IP, ELISA
Cat No. 83619-5-PBS

产品说明书

CloneNo. 240534C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

240534C7, CUL-3, Cullin-3, KIAA0617

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CUL3 Ready-To-Use IHC Kit. CUL3 primary antibody included.

产品信息

83619-5-PBS targets CUL3 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 CUL3 fusion protein Ag1555 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cullin 3
别名 240534C7, CUL-3, Cullin-3, KIAA0617
计算分子量 89 kDa
观测分子量 80-89 kDa
GenBank蛋白编号BC039598
基因名称 CUL3
Gene ID (NCBI) 8452
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ13618
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.