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验证数据展示

  • WB analysis of U2OS using 84953-4-RR

    WB analysis of U2OS using 84953-4-RR

    U2OS cells were subjected to SDS PAGE followed by western blot with 84953-4-RR (CSTA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 84953-4-RR

    IHC staining of human stomach cancer using 84953-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84953-4-RR (Cystatin A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 84953-4-RR

    IHC staining of human stomach cancer using 84953-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84953-4-RR (Cystatin A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84953-4-RR

    Affinity and Kinetic Characterization of 84953-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84953-4-RR against Human Cystatin A were performed. The affinity constant is 0.286 nM.

Cystatin A Recombinant monoclonal antibody

Cystatin A Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84953-4-RR

产品说明书

CloneNo. 242558F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CSTA, 242558F10, Cystatin AS, Cystatin-A, Cystatin-A, N-terminally processed

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84953-4-RR targets Cystatin A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8733

Product name: Recombinant human CSTA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-98 aa of BC010379

Sequence: MIPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVFKSLPGQNEDLVLTGYQVDKNKDDELTGF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cystatin A (stefin A)
别名 CSTA, 242558F10, Cystatin AS, Cystatin-A, Cystatin-A, N-terminally processed
计算分子量 98 aa, 11 kDa
观测分子量11 kDa
GenBank蛋白编号BC010379
基因名称 Cystatin A
Gene ID (NCBI) 1475
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01040
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin A (CSTA) is a member of the cystatin superfamily, which are thiol proteinase inhibitors (TPI) that play crucial roles in regulating proteolytic activity. It is a small protein composed of 98 amino acids with a molecular weight of approximately 11 kDa. CSTA is primarily expressed in epithelial tissues, such as the skin and esophagus, where it serves as a precursor protein for the cornified cell envelope in keratinocytes.

实验方案

Product Specific Protocols
IHC protocol for Cystatin A antibody 84953-4-RRDownload protocol
WB protocol for Cystatin A antibody 84953-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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