验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84477-6-RR targets CSF1R/CD115 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | colony stimulating factor 1 receptor |
| 别名 | CSF1R, CSF-1 receptor, CSF 1 R, CD115, C FMS |
| 计算分子量 | 972 aa, 108 kDa |
| 观测分子量 | 160 kDa |
| GenBank蛋白编号 | BC047521 |
| 基因名称 | CSF1R/CD115 |
| Gene ID (NCBI) | 1436 |
| RRID | AB_3671998 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P07333 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CSF1R/CD115 antibody 84477-6-RR | Download protocol |
| WB protocol for CSF1R/CD115 antibody 84477-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
