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验证数据展示

  • WB analysis using 22277-1-AP (same clone as 22277-1-PBS)

    WB analysis using 22277-1-AP (same clone as 22277-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 22277-1-AP (CREBBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 22277-1-AP (same clone as 22277-1-PBS)

    IHC staining of human prostate cancer using 22277-1-AP (same clone as 22277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CREBBP antibody included
  • IHC staining of human prostate cancer using 22277-1-AP (same clone as 22277-1-PBS)

    IHC staining of human prostate cancer using 22277-1-AP (same clone as 22277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CREBBP antibody included
  • IF Staining of HeLa using 22277-1-AP (same clone as 22277-1-PBS)

    IF Staining of HeLa using 22277-1-AP (same clone as 22277-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREBBP antibody (22277-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 22277-1-AP (same clone as 22277-1-PBS)

    FC experiment of HeLa using 22277-1-AP (same clone as 22277-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CREBBP Polyclonal antibody (22277-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

CREBBP Polyclonal antibody, PBS Only

CREBBP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22277-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CBP, KAT3A, MKHK1, RSTS, RSTS1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CREBBP Ready-To-Use IHC Kit. CREBBP primary antibody included.

产品信息

22277-1-PBS targets CREBBP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CREB binding protein
别名 CBP, KAT3A, MKHK1, RSTS, RSTS1
计算分子量 265 kDa
观测分子量290 kDa
GenBank蛋白编号NM_004380
基因名称 CREBBP
Gene ID (NCBI) 1387
RRIDAB_3085692
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92793
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The acetyltransferase CREB-binding protein (CREBBP; also known as CBP or KAT2A) and E1A-binding protein P300 (EP300; also known as P300 or KAT2B) are two closely related regulators. Both are widely expressed within and outside the hematopoietic system and serve as tumor activators or suppressors depending on the situation. CREBBP/EP300 regulate various key physiological functions, including cell apoptosis, proliferation and differentiation, DNA repair, and somatic cell reprogramming.

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