验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
16440-1-AP targets Carboxypeptidase M in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag9761 Product name: Recombinant human CPM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 99-426 aa of BC022276 Sequence: YLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPFDNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNNKASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDSIPVSNPSCPMIPLYRNLPDHSAAT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | carboxypeptidase M |
| 别名 | Carboxypeptidase M, CPM |
| 计算分子量 | 443 aa, 51 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC022276 |
| 基因名称 | Carboxypeptidase M |
| Gene ID (NCBI) | 1368 |
| RRID | AB_2084046 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14384 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CPM(Carboxypeptidase M), a plasma membrane-bound enzyme, is present in many human organs and differs from other carboxypeptidases that cleave basic C-terminal amino acids(PMID:2394713). CPM is structurally, catalytically and immunologically distinct from other carboxypeptidases, and expressed on cell membranes in a glycosyl-phosphatidylinositol-anchored form and it can be a novel marker and cellular player in lipid uptake and/or metabolism of MAs by promoting foam cell formation (PMID:22157720). This full length protein has a signal peptide with 17 amino acid, a propeptide with 20 amino acid and five glycosylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Carboxypeptidase M antibody 16440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
