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验证数据展示

  • WB analysis using 83822-2-RR

    WB analysis using 83822-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83822-2-RR (COPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 83822-2-RR

    WB analysis of HeLa using 83822-2-RR

    WB result of COPS3 antibody (83822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COPS3 transfected HeLa cells.

  • IHC staining of mouse brain using 83822-2-RR

    IHC staining of mouse brain using 83822-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83822-2-RR (COPS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPS3 antibody included
  • IF Staining of rat brain using 83822-2-RR

    IF Staining of rat brain using 83822-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 83822-2-RR

    IF Staining of A431 using 83822-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83822-2-RR

    Affinity and Kinetic Characterization of 83822-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83822-2-RR against Human COPS3 were performed. The affinity constant is 1.29 nM.

  • Featured Product
  • KD/KO验证
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COPS3 Recombinant monoclonal antibody

COPS3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 83822-2-RR

产品说明书

CloneNo. 240918E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

240918E3, COP 9 signalosome complex subunit 3, JAB1-containing signalosome subunit 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HT-29 cells, HEK-293T cells, mouse ovary tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inA431 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy COPS3 Ready-To-Use IHC Kit. COPS3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83822-2-RR targets COPS3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32825

Product name: Recombinant human COPS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 270-423 aa of BC001891

Sequence: NNPSELRNLVNKHSETFTRDNNMGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQFVQKSMGSQEDDSGNKPSSYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
别名 240918E3, COP 9 signalosome complex subunit 3, JAB1-containing signalosome subunit 3
计算分子量 48 kDa
观测分子量46 kDa
GenBank蛋白编号BC001891
基因名称 COPS3
Gene ID (NCBI) 8533
RRIDAB_3671406
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UNS2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

实验方案

Product Specific Protocols
WB protocol for COPS3 antibody 83822-2-RRDownload protocol
IHC protocol for COPS3 antibody 83822-2-RRDownload protocol
IF protocol for COPS3 antibody 83822-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols