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验证数据展示

  • WB analysis of A375 using 83010-1-RR (same clone as 83010-1-PBS)

    WB analysis of A375 using 83010-1-RR (same clone as 83010-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 83010-1-RR (COL6A3-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83010-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83010-1-RR (same clone as 83010-1-PBS)

    FC experiment of HeLa using 83010-1-RR (same clone as 83010-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug COL6A3-Specific Recombinant antibody (83010-1-RR, Clone:230540A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83010-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00027-3

    Cytometric bead array standard curve of MP00027-3

    Cytometric bead array standard curve of MP00027-3, COL6A3-Specific Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83010-1-PBS. Detection antibody: 83010-4-PBS. Standard: Ag34752. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83010-1-RR

    Affinity and Kinetic Characterization of 83010-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83010-1-RR against Human COL6A3-Specific were performed. The affinity constant is 1.63 nM.

COL6A3 Recombinant antibody, PBS Only (Capture)

COL6A3 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83010-1-PBS

产品说明书

CloneNo. 230540A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

COL6A3-Specific, 230540A11, Collagen alpha 3(VI) chain, Collagen alpha-3(VI) chain, Collagen Type VI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83010-1-PBS targets COL6A3 as part of a matched antibody pair:

MP00027-3: 83010-1-PBS capture and 83010-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 COL6A3 fusion protein Ag34752 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type VI, alpha 3
别名 COL6A3-Specific, 230540A11, Collagen alpha 3(VI) chain, Collagen alpha-3(VI) chain, Collagen Type VI
计算分子量 344 kDa
观测分子量300 kDa
GenBank蛋白编号NM_004369
基因名称 COL6A3
Gene ID (NCBI) 1293
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12111
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.