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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68358-1-Ig (same clone as 68358-1-PBS)

    WB analysis using 68358-1-Ig (same clone as 68358-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68358-1-Ig (COASY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 68358-1-Ig (same clone as 68358-1-PBS)

    IHC staining of human liver using 68358-1-Ig (same clone as 68358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68358-1-Ig (COASY antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68358-1-Ig (same clone as 68358-1-PBS)

    FC experiment of HeLa using 68358-1-Ig (same clone as 68358-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug COASY Monoclonal antibody (68358-1-Ig, Clone:1D1C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

COASY Monoclonal antibody, PBS Only

COASY Monoclonal Antibody for Indirect ELISA, IHC, WB
Cat No. 68358-1-PBS

产品说明书

CloneNo. 1D1C11

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

Indirect ELISA, IHC, WB

别名

Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase, CoA synthase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68358-1-PBS targets COASY in Indirect ELISA, IHC, WB applications and shows reactivity with rat, mouse, human samples.

经测试应用 Indirect ELISA, IHC, WB Application Description
经测试反应性 rat, mouse, human
免疫原 COASY fusion protein Ag33142 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Coenzyme A synthase
别名 Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase, CoA synthase
计算分子量 564 aa, 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC020985
基因名称 COASY
Gene ID (NCBI) 80347
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13057
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the fourth and fifth sequential steps of the CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. It was reported that having a key role in erythroid maturation and identified upstream and downstream metabolites of COASY as a potential treatment for anemia in patients with MDS-RS (PMID: 36857430). COASY has 2 isoforms with molecular weights of 62 and 65 kDa.