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验证数据展示

  • WB analysis using 86550-3-RR (same clone as 86550-3-PBS)

    WB analysis using 86550-3-RR (same clone as 86550-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86550-3-RR (CNN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86550-3-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 86550-3-RR (same clone as 86550-3-PBS)

    IP experiment of HEK-293 using 86550-3-RR (same clone as 86550-3-PBS)

    IP result of anti-CNN3 (IP:86550-3-RR, 4ug; Detection:86550-3-RR 1:2000) with HEK-293 cells lysate 1200 ug. This data was developed using the same antibody clone with 86550-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86550-3-RR (same clone as 86550-3-PBS)

    IF Staining of HeLa using 86550-3-RR (same clone as 86550-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CNN3 antibody (86550-3-RR, Clone: 251475E9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86550-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86550-3-RR

    Affinity and Kinetic Characterization of 86550-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86550-3-RR against Human CNN3 were performed. The affinity constant is 1.83 nM.

CNN3 Recombinant monoclonal antibody, PBS Only

CNN3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 86550-3-PBS

产品说明书

CloneNo. 251475E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

Calponin 3, Calponin3, Calponin-3, CNN 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CNN3 Ready-To-Use IHC Kit. CNN3 primary antibody included.

产品信息

86550-3-PBS targets CNN3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2068

Product name: Recombinant human CNN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-329 aa of BC025372

Sequence: MTHFNKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYPRDYQYSDQGIDY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calponin 3, acidic
别名 Calponin 3, Calponin3, Calponin-3, CNN 3
计算分子量 329 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC025372
基因名称 CNN3
Gene ID (NCBI) 1266
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15417
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain. CNN3 is the thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction.

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