验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Jurkat cells, MDA-MB-231 cells, U-251 cells, mouse brain tissue, U-87 MG cellls, SK-BR-3 cells |
| Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83354-3-RR targets CNKSR2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag21174 Product name: Recombinant human CNKSR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 400-558 aa of BC126121 Sequence: RKRFNIVEEDTVLYCYEYEKGRSSSQGRRESTPTYGKLRPISMPVEYNWVGDYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKKDTGKKSKKKGDKSNSPTHYSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGPIAGK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | connector enhancer of kinase suppressor of Ras 2 |
| 别名 | KSR2, KIAA0902, Connector enhancer of KSR 2, CNK2, CNK homolog protein 2 |
| 计算分子量 | 1034 aa, 118 kDa |
| 观测分子量 | 118 kDa, 102 kDa |
| GenBank蛋白编号 | BC126121 |
| 基因名称 | CNKSR2 |
| Gene ID (NCBI) | 22866 |
| RRID | AB_3671011 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8WXI2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CNKSR2 is a multidomain protein that functions as a scaffold protein to mediate the mitogen-activated protein kinase pathways downstream from Ras. This gene product is induced by vitamin D and inhibits apoptosis in certain cancer cells. It may also play a role in ternary complex assembly of synaptic proteins at the postsynaptic membrane and coupling of signal transduction to membrane/cytoskeletal remodeling.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNKSR2 antibody 83354-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |