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验证数据展示

  • WB analysis of rat pancreas using 84224-2-RR

    WB analysis of rat pancreas using 84224-2-RR

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 84224-2-RR (CLPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 84224-2-RR

    IHC staining of mouse pancreas using 84224-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 84224-2-RR

    IHC staining of mouse pancreas using 84224-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84224-2-RR

    Affinity and Kinetic Characterization of 84224-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84224-2-RR against Human CLPS were performed. The affinity constant is below 1 pM.

CLPS Recombinant antibody

CLPS Recombinant Antibody for WB, IHC, ELISA
Cat No. 84224-2-RR

产品说明书

CloneNo. 241565H3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Colipase, 241565H3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84224-2-RR targets CLPS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLPS fusion protein Ag2321 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colipase, pancreatic
别名 Colipase, 241565H3
计算分子量 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC017897
基因名称 CLPS
Gene ID (NCBI) 1208
RRIDAB_3671777
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04118
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.

实验方案

Product Specific Protocols
WB protocol for CLPS antibody 84224-2-RRDownload protocol
IHC protocol for CLPS antibody 84224-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols