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验证数据展示

  • WB analysis using 84327-1-RR

    WB analysis using 84327-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84327-1-RR (CLEC14A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84327-1-RR

    IHC staining of human ovary cancer using 84327-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84327-1-RR (CLEC14A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 84327-1-RR

    IHC staining of human placenta using 84327-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84327-1-RR (CLEC14A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84327-1-RR

    Affinity and Kinetic Characterization of 84327-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84327-1-RR against Human CLEC14A were performed. The affinity constant is 8.76 nM.

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CLEC14A Recombinant monoclonal antibody

CLEC14A Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84327-1-RR

产品说明书

CloneNo. 241206D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

241206D3, C14orf27, CEG1, C-type lectin domain family 14 member A, EGFR-5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HUVEC cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman placenta tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84327-1-RR targets CLEC14A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21081

Product name: Recombinant human CLEC14A protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-349 aa of BC031567

Sequence: MRPAFALCLLWQALWPGPGGGEHPTADRAGCSASGACYSLHHATMKRQAAEEACILRGGALSTVRAGAELRAVLALLRAGPGPGGGSKDLLFWVALERRRSHCTLENEPLRGFSWLSSDPGGLESDTLQWVEEPQRSCTARRCAVLQATGGVEPAGWKEMRCHLRANGYLCKYQFEVLCPAPRPGAASNLSYRAPFQLHSAALDFSPPGTEVSALCRGQLPISVTCIADEIGARWDKLSGDVLCPCPGRYLRAGKCAELPNCLDDLGGFACECATGFELGKDGRSCVTSGEGQPTLGGTGVPTRRPPATATSPVPQRTWPIRVDEKLGETPLVPEQDNSVTSIPEIPRW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 C-type lectin domain family 14, member A
别名 241206D3, C14orf27, CEG1, C-type lectin domain family 14 member A, EGFR-5
计算分子量 490 aa, 52 kDa
观测分子量 52 kDa, 100 kDa
GenBank蛋白编号BC031567
基因名称 CLEC14A
Gene ID (NCBI) 161198
RRIDAB_3671868
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ86T13
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

实验方案

Product Specific Protocols
WB protocol for CLEC14A antibody 84327-1-RRDownload protocol
IHC protocol for CLEC14A antibody 84327-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols