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验证数据展示

  • WB analysis using 84327-1-RR

    WB analysis using 84327-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84327-1-RR (CLEC14A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84327-1-RR

    IHC staining of human ovary cancer using 84327-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84327-1-RR (CLEC14A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 84327-1-RR

    IHC staining of human placenta using 84327-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84327-1-RR (CLEC14A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84327-1-RR

    Affinity and Kinetic Characterization of 84327-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84327-1-RR against Human CLEC14A were performed. The affinity constant is 8.76 nM.

CLEC14A Recombinant antibody

CLEC14A Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84327-1-RR

产品说明书

CloneNo. 241206D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

241206D3, C14orf27, CEG1, C-type lectin domain family 14 member A, EGFR-5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HUVEC cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman placenta tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84327-1-RR targets CLEC14A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLEC14A fusion protein Ag21081 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 C-type lectin domain family 14, member A
别名 241206D3, C14orf27, CEG1, C-type lectin domain family 14 member A, EGFR-5
计算分子量 490 aa, 52 kDa
观测分子量 52 kDa, 100 kDa
GenBank蛋白编号BC031567
基因名称 CLEC14A
Gene ID (NCBI) 161198
RRIDAB_3671868
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ86T13
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

实验方案

Product Specific Protocols
WB protocol for CLEC14A antibody 84327-1-RRDownload protocol
IHC protocol for CLEC14A antibody 84327-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols