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验证数据展示

  • IF Staining of HeLa using 84865-4-RR

    IF Staining of HeLa using 84865-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 84865-4-RR

    IF Staining of A431 using 84865-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84865-4-RR

    Affinity and Kinetic Characterization of 84865-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84865-4-RR against Human CENPF were performed. The affinity constant is 11.1 nM.

CENPF Recombinant monoclonal antibody

CENPF Uni-rAb® Recombinant Antibody for IF/ICC, ELISA
Cat No. 84865-4-RR

产品说明书

CloneNo. 242277G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

242277G9, CENF, CENP-F, PRO1779

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, A431 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84865-4-RR targets CENPF in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29665

Product name: Recombinant human CENPF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3011-3210 aa of NM_016343

Sequence: SVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 centromere protein F, 350/400ka (mitosin)
别名 242277G9, CENF, CENP-F, PRO1779
计算分子量 368 kDa
GenBank蛋白编号NM_016343
基因名称 CENPF
Gene ID (NCBI) 1063
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP49454
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

实验方案

Product Specific Protocols
IF protocol for CENPF antibody 84865-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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