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验证数据展示

  • WB analysis using 83105-6-RR

    WB analysis using 83105-6-RR

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83105-6-RR

    WB analysis using 83105-6-RR

    human saliva were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 83105-6-RR

    IHC staining of human tonsillitis using 83105-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83105-6-RR (CEACAM8/CD66b antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 83105-6-RR

    IHC staining of human tonsillitis using 83105-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83105-6-RR (CEACAM8/CD66b antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Transfected CHO-K1 using 83105-6-RR

    IF Staining of Transfected CHO-K1 using 83105-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Transfected CHO-K1 cells using CEACAM8 antibody (83105-6-RR, Clone: 242954B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83105-6-RR

    Affinity and Kinetic Characterization of 83105-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83105-6-RR against Human CEACAM8 were performed. The affinity constant is 14.1 nM.

CEACAM8/CD66b Recombinant monoclonal antibody

CEACAM8/CD66b Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83105-6-RR

产品说明书

CloneNo. 242954B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman saliva, human peripheral blood leukocyte
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTransfected CHO-K1

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83105-6-RR targets CEACAM8/CD66b in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg32133

Product name: Recombinant Human CEACAM-8 protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 35-319 aa of BC026263

Sequence: QLTIEAVPSNAAEGKEVLLLVHNLPQDPRGYNWYKGETVDANRRIIGYVISNQQITPGPAYSNRETIYPNASLLMRNVTRNDTGSYTLQVIKLNLMSEEVTGQFSVHPETPKPSISSNNSNPVEDKDAVAFTCEPETQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLLSVTRNDVGPYECEIQNPASANFSDPVTLNVLYGPDAPTISPSDTYYHAGVNLNLSCHAASNPPSQYSWSVNGTFQQYTQKLFIPNITTKNSGSYACHTTNSATGRNRTTVRMITVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 8
别名 CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b
计算分子量 38 kDa
观测分子量95-100kDa
GenBank蛋白编号BC026263
基因名称 CEACAM8
Gene ID (NCBI) 1088
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP31997
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, CGM6 or NCA-95, is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAM8 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes (PMID: 8104998; 18056392). It is involved in regulating adhesion and activation of eosinophils (PMID: 18056392).

实验方案

Product Specific Protocols
IF protocol for CEACAM8/CD66b antibody 83105-6-RRDownload protocol
IHC protocol for CEACAM8/CD66b antibody 83105-6-RRDownload protocol
WB protocol for CEACAM8/CD66b antibody 83105-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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