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验证数据展示

  • IP experiment of mouse brain using 12973-1-AP (same clone as 12973-1-PBS)

    IP experiment of mouse brain using 12973-1-AP (same clone as 12973-1-PBS)

    IP result of anti-CDKL5 (IP:12973-1-AP, 4ug; Detection:12973-1-AP 1:300) with mouse brain tissue lysate 3200 ug. This data was developed using the same antibody clone with 12973-1-PBS in a different storage buffer formulation.

  • WB analysis using 12973-1-AP (same clone as 12973-1-PBS)

    WB analysis using 12973-1-AP (same clone as 12973-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12973-1-AP (CDKL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12973-1-PBS in a different storage buffer formulation.

  • WB analysis using 12973-1-AP (same clone as 12973-1-PBS)

    WB analysis using 12973-1-AP (same clone as 12973-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12973-1-AP (CDKL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12973-1-PBS in a different storage buffer formulation.

CDKL5 Polyclonal antibody, PBS Only

CDKL5 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12973-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

cyclin dependent kinase like 5, Cyclin-dependent kinase-like 5, EC:2.7.11.22, ISSX, Serine/threonine-protein kinase 9

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12973-1-PBS targets CDKL5 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3531

Product name: Recombinant human CDKL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC036091

Sequence: MKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYTEYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLMKNLLKLDPADRYLTEQCLNHPTFQTQR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase-like 5
别名 cyclin dependent kinase like 5, Cyclin-dependent kinase-like 5, EC:2.7.11.22, ISSX, Serine/threonine-protein kinase 9
计算分子量 1030 aa, 114 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC036091
基因名称 CDKL5
Gene ID (NCBI) 6792
RRIDAB_2877899
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76039
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin dependent kinase like 5 (CDKL5) may regulate ciliogenesis and neuronal morphogenesis. It has been reported that CDKL5 is associated with epileptic seizures, severe intellectual disability, and autistic features. The CDKL5 protein is a kinase that regulates key phosphorylation events vital to the development of the complex neuronal network of the brain.

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