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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 84617-7-RR (same clone as 84617-7-PBS)

    WB analysis of Jurkat using 84617-7-RR (same clone as 84617-7-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 84617-7-RR (same clone as 84617-7-PBS)

    WB analysis of Raji using 84617-7-RR (same clone as 84617-7-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

  • WB analysis of U-87 MG using 84617-7-RR (same clone as 84617-7-PBS)

    WB analysis of U-87 MG using 84617-7-RR (same clone as 84617-7-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84617-7-RR (same clone as 84617-7-PBS)

    IHC staining of human ovary cancer using 84617-7-RR (same clone as 84617-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84617-7-RR (same clone as 84617-7-PBS)

    IHC staining of human tonsillitis using 84617-7-RR (same clone as 84617-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84617-7-RR

    Affinity and Kinetic Characterization of 84617-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84617-7-RR against Human CD82 were performed. The affinity constant is below 1 pM.

CD82 Recombinant antibody, PBS Only

CD82 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84617-7-PBS

产品说明书

CloneNo. 241765G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84617-7-PBS targets CD82 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD82 molecule
别名 CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82
计算分子量30 kDa
观测分子量35-45 kDa
GenBank蛋白编号NM_002231.4
基因名称 CD82
Gene ID (NCBI) 3732
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP27701-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.