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验证数据展示

  • WB analysis using 84234-5-RR

    WB analysis using 84234-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84234-5-RR (CD79b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84234-5-RR

    IHC staining of human tonsillitis using 84234-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84234-5-RR (CD79b antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84234-5-RR

    IHC staining of human tonsillitis using 84234-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84234-5-RR (CD79b antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Raji using 84234-5-RR

    IF Staining of Raji using 84234-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD79b antibody (84234-5-RR, Clone: 241273B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84234-5-RR

    Affinity and Kinetic Characterization of 84234-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84234-5-RR against Human CD79b were performed. The affinity constant is 3.23 nM.

CD79b Recombinant antibody

CD79b Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84234-5-RR

产品说明书

CloneNo. 241273B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

241273B7, B29, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig beta

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, Ramos cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84234-5-RR targets CD79b in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD79b molecule, immunoglobulin-associated beta
别名 241273B7, B29, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig beta
计算分子量 229 aa, 26 kDa
观测分子量32-36 kDa
GenBank蛋白编号BC032651
基因名称 CD79B
Gene ID (NCBI) 974
RRIDAB_3671787
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP40259
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).

实验方案

Product Specific Protocols
WB protocol for CD79b antibody 84234-5-RRDownload protocol
IHC protocol for CD79b antibody 84234-5-RRDownload protocol
IF protocol for CD79b antibody 84234-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols