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验证数据展示

  • WB analysis using 84596-4-RR

    WB analysis using 84596-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84596-4-RR (CD68 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84596-4-RR

    IHC staining of human tonsillitis using 84596-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of human tonsillitis using 84596-4-RR

    IHC staining of human tonsillitis using 84596-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IF Staining of human tonsillitis using 84596-4-RR

    IF Staining of human tonsillitis using 84596-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD68 antibody (84596-4-RR, Clone: 241810B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD68 Recombinant monoclonal antibody

CD68 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84596-4-RR

产品说明书

CloneNo. 241810B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

241810B3, CD68 molecule, GP110, Macrosialin, SCARD1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJ774A.1 cells, U-937 cells, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84596-4-RR targets CD68 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg2061

Product name: Recombinant Human CD68 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 22-319 aa of BC015557

Sequence: NDCPHKKSATLLPSFTVTPTVTESTGTTSHRTTKSHKTTTHRTTTTGTTSHGPTTATHNPTTTSHGNVTVHPTSNSTATSQGPSTATHSPATTSHGNATVHPTSNSTATSPGFTSSAHPEPPPPSPSPSPTSKETIGDYTWTNGSQPCVHLQAQIQIRVMYTTQGGGEAWGISVLNPNKTKVQGSCEGAHPHLLLSFPYGHLSFGFMQDLQQKVVYLSYMAVEYNVSFPHAAQWTFSAQNASLRDLQAPLGQSFSCSNSSIILSPAVHLDLLSLRLQAAQLPHTGVFGQSFSCPSDRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD68 molecule
别名 241810B3, CD68 molecule, GP110, Macrosialin, SCARD1
计算分子量 37 kDa
观测分子量80-90 kDa
GenBank蛋白编号BC015557
基因名称 CD68
Gene ID (NCBI) 968
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP34810
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in the phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
WB protocol for CD68 antibody 84596-4-RRDownload protocol
IHC protocol for CD68 antibody 84596-4-RRDownload protocol
IF protocol for CD68 antibody 84596-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols