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验证数据展示

  • WB analysis of Jurkat using 84405-6-RR

    WB analysis of Jurkat using 84405-6-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 84405-6-RR (CD45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 84405-6-RR

    IF Staining of Jurkat using 84405-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45(220-577) antibody (84405-6-RR, Clone: 241670B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of Raji using 84405-6-RR

    IF Staining of Raji using 84405-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD45(220-577) antibody (84405-6-RR, Clone: 241670B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human tonsillitis using 84405-6-RR

    IHC staining of human tonsillitis using 84405-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84405-6-RR (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD45 antibody included
  • IHC staining of human tonsillitis using 84405-6-RR

    IHC staining of human tonsillitis using 84405-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84405-6-RR (CD45 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD45 antibody included
  • Affinity and Kinetic Characterization of 84405-6-RR

    Affinity and Kinetic Characterization of 84405-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84405-6-RR against Human CD45 were performed. The affinity constant is 3.91 nM.

CD45 Recombinant monoclonal antibody

CD45 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84405-6-RR

产品说明书

CloneNo. 241670B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

241670B6, EC:3.1.3.48, GP180, L CA, LCA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells, Raji cells
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84405-6-RR targets CD45 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2486

Product name: recombinant human CD45(220-577) protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10007019 of

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 241670B6, EC:3.1.3.48, GP180, L CA, LCA
计算分子量147 kDa
观测分子量220-245 kDa
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_3671934
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP08575
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
IF protocol for CD45 antibody 84405-6-RRDownload protocol
IHC protocol for CD45 antibody 84405-6-RRDownload protocol
WB protocol for CD45 antibody 84405-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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